Home
UTas
Biophysics Lab
Shabala Lab
MIFE Research Facility
MIFE user group
Overview
Membrane
Transport & fluxes
MIFE
Key Features
MIFE applications
development
references
MIFE theory
ion flux
theory
multivalent ion
mobility
ionic mobility values
neutral
molecule flux
Methodological isues
H+ flux in buffered media
Hardware
amplifier
microscope
manipulator
System requirements
system
components
Software
CHART
MIFEFLUX
analysis
Purchasing
Univ
of Tas
Eppendorf NP2
|
Overview of the MIFE system - useful references
Papers highlighted in red
are central to the technique.
A definitive
review of the technique and its applications was published in January
2001. Gilliham et al. have powerfully linked MIFE
with whole cell patch clamp.
-
Ammann, D. (1986) Ion-selective microelectrodes.
Springer-Verlag, Berlin Heidelberg New York Tokyo
-
Arif I. & Newman I.A. (1993) Proton
efflux from oat coleoptile cells and exchange with wall calcium after IAA
or fusicoccin treatment. Planta. 189, 377-383.
-
Arif I., Newman I.A. & Keenlyside
N. (1995) Proton flux measurement from tissues in buffered solution. Plant,
Cell & Environment. 18, 1319-1324.
-
Gilliham G, Sullivan W,
Tester M, Tyerman S (2006) Simultaneuos flux and current measurements from
single plant protoplasts reveals a strong link between K+ fluxes and
current, but no link between Ca2+ fluxes and current. The Plant
Journal 46, 134-144. ABSTRACT.
-
Henriksen G.H., Bloom A.J. & Spanswick
R.M. (1990) Measurement of net fluxes of ammonium and nitrate at the surface
of barley roots using ion-selective microelectrodes. Plant Physiology 93,
271-280.
-
Henriksen G.H., Raman D.R., Walker
L.P. & Spanswick R.M. (1992) Measurement of net fluxes of ammonium
and nitrate at the surface of barley roots using ion-selective microelectrodes.
II. Patterns of uptake along the root axis and evaluation of the microelectrode
flux estimation technique. Plant Physiology 99, 734-747.
-
Huang J.W., Grunes D.L. & Kochian
L. (1992) Aluminium effects on the kinetics of calcium uptake into cells
of the wheat root apex. Quantification of calcium fluxes using a calcium-selective
vibrating microelectrode. Planta 188, 414-421.
-
Hush J.M., Newman I.A. & Overall
R.L. (1992) Utilizition of the vibrating probe and ion-selective microelectrode
techniques to investigate electrophysiological responses to wounding in
pea roots. Journal of Experimental Botany 43, 1251-1257.
-
Hush J M, Overall R L & Newman
I A (1991) A calcium influx precedes organogenesis in Graptopetalum. Plant
Cell & Environment 14, 657-665.
-
Kochian, L V, Shaff J E, Kuhtreiber
W M, Jaffe L F & Lucas W J (1992) Use of an extracellular, ion-selective
microelectrode system for the quantification of K+, H+,
and Ca2+ fluxes in maize roots and maize suspension cells. Planta
188, 601-610.
-
Kuhtreiber W.M. and Jaffe L.F. (1990)
Detection of extracellular calcium gradients with a calcium- specific vibrating
electrode. J Cell Biol 110, 1565-1573.
-
Lucas W.J. & Kochian L.V. (1986)
Ion transport processes in corn roots: an appr oach utilising microelectrode
techniques. In Advanced Agricultural Instrumentation. Design and Use. (ed.
W.G. Gensler). pp. 402-425. Martinus Nijhoff, Dordrecht.
-
Newman I.A. (2001)
Ion transport in roots: measurement of fluxes using ion-selective microelectrodes
to characterize transporter function. Plant, Cell & Environment
24, 1-14. REVIEW ABSTRACT.
-
Newman I.A., Kochian L.V., Grusak M.A.
& Lucas W.J. (1987) Fluxes of H+ and K+ in corn
roots. Characterization and stoichiometries using ion selective microelectrodes.
Plant Physiology 84, 1177-1184.
-
Pierson ES. Smith PJS. Shipley AM.
Jaffe LF. Cresti M. Hepler PK. (1993) Ca2+ fluxes around pollen
grains and pollen tubes of lily - normal development and effects of thermal
shock, BAPTA-type buffer microinjection and depletion of boric acid from
the medium. Biological Bulletin. 185(2), 302-303.
-
Ryan P.R., Newman I.A. & Arif I.
(1992) Rapid calcium exchange for protons and potassium in cell walls of
Chara. Plant Cell and Environment 15, 675-683.
-
Ryan P.R., Newman I.A. & Shields
B. (1990) Ion fluxes in corn roots measured by microelectrodes with ion-specific
liquid membranes. Journal of Membrane Science 53, 59-69.
-
Shabala S.N., Newman I.A. & Morris
J. (1997) Oscillations in H+ and Ca2+ ion fluxes
around the elongation region of corn roots and effects of external pH.
Plant Physiology 113, 111-118.
-
Shabala S.N., Newman I.A. (1997) Proton
and calcium flux oscillations in the elongation region correlate with root
nutation. Physiologia Plantarum 100(4), 917-926.
-
Shabala SN & Newman IA (1997) H+ flux kinetics
around plant roots after short-term exposure to low temperature: identifying
critical temperatures for plant chilling tolerance. Plant Cell &
Environment 20, 1401-1410.
-
Shabala SN, Newman IA, Whittington J & Juswono UP (1998)
Protoplast ion fluxes: their measurements and variation with time, position
and osmoticum. Planta 204, 146-152.
-
Shabala SN & Newman IA (1998) Osmotic sensitivity of
Ca2+ and H+ transporters in corn roots: Effect on
fluxes and their oscillations in the elongating region. The Journal
of Membrane Biology 161, 45-54.
-
Smith J.R. (1993) Can membrane transport
parameters that vary with time be derived from electrochemical measurements
in an external electrolyte? Journal of the Chemical Society- Faraday Transactions.
89(15), 2783-2789.
-
Walker D.J., Smith S.J. and Miller A.J. (1995) Simultaneous measurement of intracellular pH and K+
or NO3- in barley root cells using triple-barrelled,
ion-selective microelectrodes. Plant Physiology 108,743-751.
|